Visualization and exploration of Tcf/Lef function using a highly responsive Wnt/Β-catenin signaling-reporter transgenic zebrafish

Nobuyuki Shimizu, Koichi Kawakami, Tohru Ishitani

研究成果: ジャーナルへの寄稿学術誌査読

99 被引用数 (Scopus)

抄録

Evolutionarily conserved Tcf/Lef transcription factors (Lef1, Tcf7, Tcf7l1, and Tcf7l2) mediate gene expression regulated by Wnt/Β-catenin signaling, which has multiple roles in early embryogenesis, organogenesis, adult tissue homeostasis, and tissue regeneration. However, the spatiotemporal dynamics of Tcf/Lef activity during these events remain poorly understood. We generated stable transgenic zebrafish lines carrying a new Wnt/Β-catenin signaling reporter, Tcf/Lef-miniP:dGFP. The reporter revealed the transcriptional activities of four Tcf/Lef members controlled by Wnt/Β-catenin signaling, which were expressed in known Wnt/Β-catenin signaling-active sites during embryogenesis, organ development and growth, and tissue regeneration. We used the transgenic lines to demonstrate the contribution of Tcf/Lef-mediated Wnt/Β-catenin signaling to the development of the anterior lateral line, dorsal and secondary posterior lateral lines, and gill filaments. Thus, these reporter lines are highly useful tools for studying Tcf/Lef-mediated Wnt/Β-catenin signaling-dependent processes.

本文言語英語
ページ(範囲)71-85
ページ数15
ジャーナルDevelopmental Biology
370
1
DOI
出版ステータス出版済み - 10月 1 2012

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 発生生物学
  • 細胞生物学

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