TY - JOUR
T1 - TRIM8 regulates Nanog via Hsp90β-mediated nuclear translocation of STAT3 in embryonic stem cells
AU - Okumura, Fumihiko
AU - Okumura, Akiko J.
AU - Matsumoto, Masaki
AU - Nakayama, Keiichi I.
AU - Hatakeyama, Shigetsugu
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology and Mitsubishi Pharma Sources of Funding (to S. Hatakeyama and F. Okumura), Ono Cancer Research Fund and the Research Foundation Ituu Laboratory (to S. Hatakeyama).
PY - 2011/10
Y1 - 2011/10
N2 - TRIM8 is a member of a protein family defined by the presence of a common domain structure composed of a tripartite motif including a RING-finger, one or two B-box domains and a coiled-coil motif. Here, we show that TRIM8 interacts with Hsp90β, which interacts with STAT3 and selectively downregulates transcription of Nanog in embryonic stem cells. Knock-down of TRIM8 increased phosphorylated STAT3 in the nucleus and also enhanced transcription of Nanog. These findings suggest that TRIM8 modulates translocation of phosphorylated STAT3 into the nucleus through interaction with Hsp90β and consequently regulates transcription of Nanog in embryonic stem cells.
AB - TRIM8 is a member of a protein family defined by the presence of a common domain structure composed of a tripartite motif including a RING-finger, one or two B-box domains and a coiled-coil motif. Here, we show that TRIM8 interacts with Hsp90β, which interacts with STAT3 and selectively downregulates transcription of Nanog in embryonic stem cells. Knock-down of TRIM8 increased phosphorylated STAT3 in the nucleus and also enhanced transcription of Nanog. These findings suggest that TRIM8 modulates translocation of phosphorylated STAT3 into the nucleus through interaction with Hsp90β and consequently regulates transcription of Nanog in embryonic stem cells.
UR - https://www.scopus.com/pages/publications/80051786234
UR - https://www.scopus.com/pages/publications/80051786234#tab=citedBy
U2 - 10.1016/j.bbamcr.2011.05.013
DO - 10.1016/j.bbamcr.2011.05.013
M3 - Article
C2 - 21689689
AN - SCOPUS:80051786234
SN - 0167-4889
VL - 1813
SP - 1784
EP - 1792
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
IS - 10
ER -