TY - JOUR
T1 - Transcriptomic features associated with energy production in the muscles of Pacific bluefin tuna and Pacific cod
AU - Shibata, Mami
AU - Mekuchi, Miyuki
AU - Mori, Kazuki
AU - Muta, Shigeru
AU - Chowdhury, Vishwajit Sur
AU - Nakamura, Yoji
AU - Ojima, Nobuhiko
AU - Saitoh, Kenji
AU - Kobayashi, Takanori
AU - Wada, Tokio
AU - Inouye, Iyoshi
AU - Kuhara, Satoru
AU - Tashiro, Suke
N1 - Funding Information:
This work was supported by funds to the FRA for general research and facility setup from the Fisheries Agency (Ministry of Agriculture, Forestry and Fisheries, Japan).
Publisher Copyright:
© 2016 Japan Society for Bioscience, Biotechnology, and Agrochemistry.
PY - 2016
Y1 - 2016
N2 - Bluefin tuna are high-performance swimmers and top predators in the open ocean. Their swimming is grounded by unique features including an exceptional glycolytic potential in white muscle, which is supported by high enzymatic activities. Here we performed high-throughput RNA sequencing (RNA-Seq) in muscles of the Pacific bluefin tuna (Thunnus orientalis) and Pacific cod (Gadus macrocephalus) and conducted a comparative transcriptomic analysis of genes related to energy production. We found that the total expression of glycolytic genes was much higher in the white muscle of tuna than in the other muscles, and that the expression of only six genes for glycolytic enzymes accounted for 83.4% of the total. These expression patterns were in good agreement with the patterns of enzyme activity previously reported. The findings suggest that the mRNA expression of glycolytic genes may contribute directly to the enzymatic activities in the muscles of tuna.
AB - Bluefin tuna are high-performance swimmers and top predators in the open ocean. Their swimming is grounded by unique features including an exceptional glycolytic potential in white muscle, which is supported by high enzymatic activities. Here we performed high-throughput RNA sequencing (RNA-Seq) in muscles of the Pacific bluefin tuna (Thunnus orientalis) and Pacific cod (Gadus macrocephalus) and conducted a comparative transcriptomic analysis of genes related to energy production. We found that the total expression of glycolytic genes was much higher in the white muscle of tuna than in the other muscles, and that the expression of only six genes for glycolytic enzymes accounted for 83.4% of the total. These expression patterns were in good agreement with the patterns of enzyme activity previously reported. The findings suggest that the mRNA expression of glycolytic genes may contribute directly to the enzymatic activities in the muscles of tuna.
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U2 - 10.1080/09168451.2016.1151341
DO - 10.1080/09168451.2016.1151341
M3 - Article
C2 - 26924100
AN - SCOPUS:84975073452
SN - 0916-8451
VL - 80
SP - 1114
EP - 1124
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 6
ER -