Transcription factor-mediated direct cellular reprogramming yields cell-type specific DNA methylation signature

Kenichi Horisawa, Shizuka Miura, Hiromitsu Araki, Fumihito Miura, Takashi Ito, Atsushi Suzuki

研究成果: ジャーナルへの寄稿学術誌査読

抄録

Direct reprogramming, inducing the conversion of one type of somatic cell into another by the forced expression of defined transcription factors, is a technology with anticipated medical applications. However, due to the many unresolved aspects of the induction mechanisms, it is essential to thoroughly analyze the epigenomic state of the generated cells. Here, we performed comparative genome-wide DNA methylation analyses of mouse embryonic fibroblasts (MEFs) and cells composing organoids formed by intestinal stem cells (ISCs) or induced ISCs (iISCs) that were directly induced from MEFs. We found that the CpG methylation state was similar between cells forming ISC organoids and iISC organoids, while they differed widely from those in MEFs. Moreover, genomic regions that were differentially methylated between ISC organoid- and iISC organoid-forming cells did not significantly affect gene expression. These results demonstrate the accuracy and safety of iISC induction, leading to the medical applications of this technology.

本文言語英語
論文番号22317
ジャーナルScientific reports
13
1
DOI
出版ステータス出版済み - 12月 2023

!!!All Science Journal Classification (ASJC) codes

  • 一般

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