TY - GEN
T1 - Toward automated analyses of migration and differentiation in cultured human embryonic stem cells
AU - Prigozhina, Natalie L.
AU - Russo, Joseph A.
AU - Pekurovsky, Alexander
AU - Kita-Matsuo, Hiroko
AU - Clancy, James W.
AU - Mercola, Mark
AU - Price, Jeffrey H.
PY - 2007
Y1 - 2007
N2 - During normal embryonic development, the cells follow a remarkably coordinated migration and differentiation program, many details of which remain a mystery. In situ automated analyses of the behaviors of human embryonic stem cells (hESCs) in culture would lead to better understanding of analogous interplay between cellular migration and differentiation in developmental models. We are developing automated tools to track hESC migration and cell lineages during differentiation into various cell types and structures. In these culture models, we report on differentiation of hESCs into cells of mesodermal lineage, endothelial blood vessel-like structures and neurons. Here we use semi-automated techniques to investigate formation of these biological units, and show "migration maps" created from time-lapse movies of H2B-GFP-expressing differentiating hESCs. We discuss the goals of fully automated image analysis for tracking cell migration and lineages.
AB - During normal embryonic development, the cells follow a remarkably coordinated migration and differentiation program, many details of which remain a mystery. In situ automated analyses of the behaviors of human embryonic stem cells (hESCs) in culture would lead to better understanding of analogous interplay between cellular migration and differentiation in developmental models. We are developing automated tools to track hESC migration and cell lineages during differentiation into various cell types and structures. In these culture models, we report on differentiation of hESCs into cells of mesodermal lineage, endothelial blood vessel-like structures and neurons. Here we use semi-automated techniques to investigate formation of these biological units, and show "migration maps" created from time-lapse movies of H2B-GFP-expressing differentiating hESCs. We discuss the goals of fully automated image analysis for tracking cell migration and lineages.
UR - http://www.scopus.com/inward/record.url?scp=36349037074&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36349037074&partnerID=8YFLogxK
U2 - 10.1109/ISBI.2007.357049
DO - 10.1109/ISBI.2007.357049
M3 - Conference contribution
AN - SCOPUS:36349037074
SN - 1424406722
SN - 9781424406722
T3 - 2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings
SP - 1104
EP - 1107
BT - 2007 4th IEEE International Symposium on Biomedical Imaging
T2 - 2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro; ISBI'07
Y2 - 12 April 2007 through 15 April 2007
ER -