Time-dependent complex formation of dendritic poly(L-lysine) with plasmid DNA and correlation with in vitro transfection efficiencies

Tatsuya Okuda, Satoru Kidoaki, Mio Ohsaki, Yoshiyuki Koyama, Kenichi Yoshikawa, Takuro Niidome, Haruhiko Aoyagi

研究成果: ジャーナルへの寄稿学術誌査読

67 被引用数 (Scopus)

抄録

Dendritic poly(L-lysine) of the 6th generation shows high transfection efficiency into several cultivated cells with low cytotoxicity. In order to understand the mechanism of complex formation with plasmid DNA, the complex was observed using atomic force microscopy. After mixing for 15 min, 1-2 μm assemblies of complexes composed of several small particles (50-200 nm) were observed. At the same time, individual small complexes of 50 to 500 nm were observed on a mica surface. After incubation for 2 h, only the large complexes were found on the mica surface. As a result of further dynamic light scattering analysis and measurement of the transfection efficiency at different time points, the transfection efficiency of KG6 was found to increase with increasing size of the DNA-complexes. This result indicates that large complexes of more than 1 μm are major species that contribute to transfection in vitro.

本文言語英語
ページ(範囲)1270-1273
ページ数4
ジャーナルOrganic and Biomolecular Chemistry
1
8
DOI
出版ステータス出版済み - 4月 21 2003
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 物理化学および理論化学
  • 有機化学

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