The reproducibility of measurements using a standardization phantom for the evaluation of fractional anisotropy (FA) derived from diffusion tensor imaging (DTI)

Objectives: It is necessary to standardize the examination procedure and diagnostic criteria of diffusion tensor imaging (DTI). Thus, the purpose of this study was to examine the reproducibility of measurements using a standardization phantom composed of different fibre materials with different fibre densities (FDs) for the evaluation of fractional anisotropy (FA) derived from DTI. Materials and methods: Two types of fibre materials wrapped in heat-shrinkable tubes were used as fibre phantoms. We designed fibre phantoms with three different FDs of each fibre material. The standardization phantom was examined using DTI protocol six times a day, and each examination session was repeated once a month for 7 consecutive months. Fibre tracking was performed by setting regions of interest in the FA map, and FA was measured in each fibre phantom. Coefficients of variation (CVs) were used to evaluate the inter-examination reproducibility of FA values. Furthermore, Bland–Altman plots were used to evaluate the intra-operator reproducibility of FA measurements. Results: All CVs for each fibre phantom were within 2% throughout the 7-month study of repeated DTI sessions. The high intra-operator reproducibility of the FA measurement was confirmed. Discussion: High reproducibility of measurements using a standardization phantom for the evaluation of FA was achieved.