The NF-κB binding site is essential for transcriptional activation of the IL-15 gene

Junji Washizu, Hitoshi Nishimura, Nobuhisa Nakamura, Yuji Nimura, Yasunobu Yoshikai

研究成果: ジャーナルへの寄稿学術誌査読

64 被引用数 (Scopus)

抄録

We cloned the 5' upstream region of IL-15 genomic DNA and examined promoter activity in macrophages stimulated with lipopolysaccharide (LPS). The 1.2 kilobase (kb) fragment of the 5' upstream region contained binding elements for LPS-inducible transcription factors such as NFIL-6 or NF-κB. Determined by luciferase assay following transient transfection in the J774A.1 macrophage cell line, the 1.2 kb of the 5' upstream region exhibited high promoter activity in response to LPS, while promoter activity was significantly reduced by the 5' deletion of 313 base pairs containing the NF-κB binding motif. Nuclear protein prepared from LPS-stimulated macrophages formed a complex with the NF-κB binding sequence of the IL-15 promoter. Taken together, the binding of nuclear protein to the NF-κB binding site is required for transcriptional activation of the IL-15 gene in LPS-stimulated macrophages.

本文言語英語
ページ(範囲)1-7
ページ数7
ジャーナルImmunogenetics
48
1
DOI
出版ステータス出版済み - 1998
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 免疫学
  • 遺伝学

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