The groESL operon of the halophilic lactic acid bacterium tetragenococcus halophila

Daisuke Fukuda; Maki Watanabe; Yuji Aso; Kenji Sonomoto; Ayaaki Ishizaki

doi:10.1271/bbb.66.1176

The groESL operon of the halophilic lactic acid bacterium tetragenococcus halophila

Daisuke Fukuda
, Maki Watanabe
, Yuji Aso
, Kenji Sonomoto
, Ayaaki Ishizaki

システム生物工学

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

4 被引用数 (Scopus)

抄録

The groESL operon of the halophilic lactic acid bacterium Tetragenococcus halophila was cloned by a PCR-based method. The molecular masses of GroES and GroEL proteins were calculated to be 10,153 and 56,893 Da, respectively. The amount of groESL mRNA was increased 3.8-fold by heat shock (45°C), and 4-fold by high NaCl (3-4 M). The Bacillus subtilis σ^A-like constitutive promoter existed in front of groES, and was used under both normal and stress (heat shock and high salinity) conditions.

本文言語	英語
ページ（範囲）	1176-1180
ページ数	5
ジャーナル	Bioscience, Biotechnology and Biochemistry
巻	66
号	5
DOI	https://doi.org/10.1271/bbb.66.1176
出版ステータス	出版済み - 2002

!!!All Science Journal Classification (ASJC) codes

医学一般

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10.1271/bbb.66.1176

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引用スタイル

@article{064b704db18044cc961ec7e1d40892b1,

title = "The groESL operon of the halophilic lactic acid bacterium tetragenococcus halophila",

abstract = "The groESL operon of the halophilic lactic acid bacterium Tetragenococcus halophila was cloned by a PCR-based method. The molecular masses of GroES and GroEL proteins were calculated to be 10,153 and 56,893 Da, respectively. The amount of groESL mRNA was increased 3.8-fold by heat shock (45°C), and 4-fold by high NaCl (3-4 M). The Bacillus subtilis σA-like constitutive promoter existed in front of groES, and was used under both normal and stress (heat shock and high salinity) conditions.",

keywords = "CIRCE, Groesl operon, Halophile, Heat shock protein, Tetragenococcus",

author = "Daisuke Fukuda and Maki Watanabe and Yuji Aso and Kenji Sonomoto and Ayaaki Ishizaki",

note = "Funding Information: This work was partly supported by the Sasakawa Scientiˆc Research Grant from The Japan Science Society.",

year = "2002",

doi = "10.1271/bbb.66.1176",

language = "English",

volume = "66",

pages = "1176--1180",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Oxford University Press",

number = "5",

}

TY - JOUR

T1 - The groESL operon of the halophilic lactic acid bacterium tetragenococcus halophila

AU - Fukuda, Daisuke

AU - Watanabe, Maki

AU - Aso, Yuji

AU - Sonomoto, Kenji

AU - Ishizaki, Ayaaki

N1 - Funding Information: This work was partly supported by the Sasakawa Scientiˆc Research Grant from The Japan Science Society.

PY - 2002

Y1 - 2002

N2 - The groESL operon of the halophilic lactic acid bacterium Tetragenococcus halophila was cloned by a PCR-based method. The molecular masses of GroES and GroEL proteins were calculated to be 10,153 and 56,893 Da, respectively. The amount of groESL mRNA was increased 3.8-fold by heat shock (45°C), and 4-fold by high NaCl (3-4 M). The Bacillus subtilis σA-like constitutive promoter existed in front of groES, and was used under both normal and stress (heat shock and high salinity) conditions.

AB - The groESL operon of the halophilic lactic acid bacterium Tetragenococcus halophila was cloned by a PCR-based method. The molecular masses of GroES and GroEL proteins were calculated to be 10,153 and 56,893 Da, respectively. The amount of groESL mRNA was increased 3.8-fold by heat shock (45°C), and 4-fold by high NaCl (3-4 M). The Bacillus subtilis σA-like constitutive promoter existed in front of groES, and was used under both normal and stress (heat shock and high salinity) conditions.

KW - CIRCE

KW - Groesl operon

KW - Halophile

KW - Heat shock protein

KW - Tetragenococcus

UR - https://www.scopus.com/pages/publications/0036562589

UR - https://www.scopus.com/pages/publications/0036562589#tab=citedBy

U2 - 10.1271/bbb.66.1176

DO - 10.1271/bbb.66.1176

M3 - Article

C2 - 12092841

AN - SCOPUS:0036562589

SN - 0916-8451

VL - 66

SP - 1176

EP - 1180

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 5

ER -

The groESL operon of the halophilic lactic acid bacterium tetragenococcus halophila

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!!!All Science Journal Classification (ASJC) codes

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