Synthesis of 8-oxoguanosine phosphoramidite and its incorporation into oligoribonucleotides

Yosuke Taniguchi, Yohei Koga, Shigeki Sasaki

研究成果: ジャーナルへの寄稿学術誌査読

1 被引用数 (Scopus)

抄録

The detailed synthetic protocol for preparation of the phosphoramidite of an oxidatively damaged ribonucleotide, 8-oxoguanosine (8-oxo-G), and its incorporation into RNA are described. The O6- and N7-bisdiphenylcarbamoyl-protected 8-oxoguanosine phosphoramidite was synthesized as a new phosphoramidite precursor unit for the synthesis of RNA. It was successfully incorporated into the RNA sequences, and the synthesized RNAs were completely deprotected with 28% aqueous ammonia solution at 55°C for 24 hr. After purification using HPLC, they were identified by MALDI-TOF mass measurement. The base-pairing properties showed that 8-oxo-G forms base pairs not only with rC or dC in anti-conformation, but also with rA in syn conformation within the RNA duplexes or RNA/DNA heteroduplexes.

本文言語英語
論文番号04.58
ジャーナルCurrent Protocols in Nucleic Acid Chemistry
SUPPL.56
DOI
出版ステータス出版済み - 2014

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 有機化学

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