Survey of solasodine-type glycoalkaloids by western blotting and ELISA using anti-solamargine monoclonal antibody

Solasodine glycosides were analyzed for the selection of higher- producing natural resources in the fruits of Solanum spp. by western blotting and ELISA using anti-solamargine monoclonal antibody (MAb). Western blotting of Solanum khasianum showed a correlation with the analysis by ELISA. The results indicated that the fruits of S. khasianum contained the highest level of solasodine glycosides, compared to other species having 53.43±3.90 μg/mg dry wt. The high level of solasodine glycosides in S. khasianum suggests that it may be suitable for commercial production.