Suppressor microRNA-145 Is epigenetically regulated by promoter hypermethylation in esophageal squamous cell carcinoma

Kazuto Harada; Yoshifumi Baba; Takatsugu Ishimoto; Keisuke Kosumi; Ryuma Tokunaga; Daisuke Izumi; Mayuko Ohuchi; Kenichi Nakamura; Yuki Kiyozumi; Junji Kurashige; Shiro Iwagami; Yuji Miyamoto; Yasuo Sakamoto; Naoya Yoshida; Eiji Oki; Masayuki Watanabe; Hideo Baba

Suppressor microRNA-145 Is epigenetically regulated by promoter hypermethylation in esophageal squamous cell carcinoma

Kazuto Harada, Yoshifumi Baba, Takatsugu Ishimoto, Keisuke Kosumi, Ryuma Tokunaga, Daisuke Izumi, Mayuko Ohuchi, Kenichi Nakamura, Yuki Kiyozumi, Junji Kurashige, Shiro Iwagami, Yuji Miyamoto, Yasuo Sakamoto, Naoya Yoshida, Eiji Oki, Masayuki Watanabe, Hideo Baba

消化管外科(2)

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

22 被引用数 (Scopus)

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Background/Aim: DNA methylation is a common epigenetic change in cancer. However, microRNA (miRNA) regulation by epigenetic alteration, especially CpG island hypermethylation, remains poorly understood in esophageal squamous cell carcinoma (ESCC). Materials and Methods: miRNAs which were up-regulated after de-methylation with 5- aza-2'-deoxycytidine (5-AZA) were analyzed using the Human miFinder 384HC miScript miRNA PCR Array. The DNA methylation level was evaluated by bisulfite-pyrosequencing assay. Results: In two of the cell lines, 20 miRNAs, including miR-145-5p, were found to be up-regulated by more than three-fold after 5-AZA treatment. The miRNA-145 promoter was significantly more hypermethylated in the cancer tissues than in matched normal adjacent esophageal epithelial mucosa (p=0.0042; paired t-test). Moreover, the miRNA-145- 5p expression levels were significantly lower in cancerous tissues (p=0.0024). Conclusion: miRNA-145 expression in ESCC seems to be regulated by hypermethylation of the miRNA-145 promoter region.

本文言語	英語
ページ（範囲）	4617-4624
ページ数	8
ジャーナル	Anticancer research
巻	35
号	9
出版ステータス	出版済み - 9月 1 2015

!!!All Science Journal Classification (ASJC) codes

腫瘍学
癌研究

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

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Harada, K., Baba, Y., Ishimoto, T., Kosumi, K., Tokunaga, R., Izumi, D., Ohuchi, M., Nakamura, K., Kiyozumi, Y., Kurashige, J., Iwagami, S., Miyamoto, Y., Sakamoto, Y., Yoshida, N., Oki, E., Watanabe, M., & Baba, H. (2015). Suppressor microRNA-145 Is epigenetically regulated by promoter hypermethylation in esophageal squamous cell carcinoma. Anticancer research, 35(9), 4617-4624.

Harada, K, Baba, Y, Ishimoto, T, Kosumi, K, Tokunaga, R, Izumi, D, Ohuchi, M, Nakamura, K, Kiyozumi, Y, Kurashige, J, Iwagami, S, Miyamoto, Y, Sakamoto, Y, Yoshida, N, Oki, E, Watanabe, M & Baba, H 2015, 'Suppressor microRNA-145 Is epigenetically regulated by promoter hypermethylation in esophageal squamous cell carcinoma', Anticancer research, vol. 35, no. 9, pp. 4617-4624.

@article{1163e83b9fbd4732ba753eaf7ba36e16,

title = "Suppressor microRNA-145 Is epigenetically regulated by promoter hypermethylation in esophageal squamous cell carcinoma",

abstract = "Background/Aim: DNA methylation is a common epigenetic change in cancer. However, microRNA (miRNA) regulation by epigenetic alteration, especially CpG island hypermethylation, remains poorly understood in esophageal squamous cell carcinoma (ESCC). Materials and Methods: miRNAs which were up-regulated after de-methylation with 5- aza-2'-deoxycytidine (5-AZA) were analyzed using the Human miFinder 384HC miScript miRNA PCR Array. The DNA methylation level was evaluated by bisulfite-pyrosequencing assay. Results: In two of the cell lines, 20 miRNAs, including miR-145-5p, were found to be up-regulated by more than three-fold after 5-AZA treatment. The miRNA-145 promoter was significantly more hypermethylated in the cancer tissues than in matched normal adjacent esophageal epithelial mucosa (p=0.0042; paired t-test). Moreover, the miRNA-145- 5p expression levels were significantly lower in cancerous tissues (p=0.0024). Conclusion: miRNA-145 expression in ESCC seems to be regulated by hypermethylation of the miRNA-145 promoter region.",

author = "Kazuto Harada and Yoshifumi Baba and Takatsugu Ishimoto and Keisuke Kosumi and Ryuma Tokunaga and Daisuke Izumi and Mayuko Ohuchi and Kenichi Nakamura and Yuki Kiyozumi and Junji Kurashige and Shiro Iwagami and Yuji Miyamoto and Yasuo Sakamoto and Naoya Yoshida and Eiji Oki and Masayuki Watanabe and Hideo Baba",

year = "2015",

month = sep,

day = "1",

language = "English",

volume = "35",

pages = "4617--4624",

journal = "Anticancer research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

number = "9",

}

TY - JOUR

T1 - Suppressor microRNA-145 Is epigenetically regulated by promoter hypermethylation in esophageal squamous cell carcinoma

AU - Harada, Kazuto

AU - Baba, Yoshifumi

AU - Ishimoto, Takatsugu

AU - Kosumi, Keisuke

AU - Tokunaga, Ryuma

AU - Izumi, Daisuke

AU - Ohuchi, Mayuko

AU - Nakamura, Kenichi

AU - Kiyozumi, Yuki

AU - Kurashige, Junji

AU - Iwagami, Shiro

AU - Miyamoto, Yuji

AU - Sakamoto, Yasuo

AU - Yoshida, Naoya

AU - Oki, Eiji

AU - Watanabe, Masayuki

AU - Baba, Hideo

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Background/Aim: DNA methylation is a common epigenetic change in cancer. However, microRNA (miRNA) regulation by epigenetic alteration, especially CpG island hypermethylation, remains poorly understood in esophageal squamous cell carcinoma (ESCC). Materials and Methods: miRNAs which were up-regulated after de-methylation with 5- aza-2'-deoxycytidine (5-AZA) were analyzed using the Human miFinder 384HC miScript miRNA PCR Array. The DNA methylation level was evaluated by bisulfite-pyrosequencing assay. Results: In two of the cell lines, 20 miRNAs, including miR-145-5p, were found to be up-regulated by more than three-fold after 5-AZA treatment. The miRNA-145 promoter was significantly more hypermethylated in the cancer tissues than in matched normal adjacent esophageal epithelial mucosa (p=0.0042; paired t-test). Moreover, the miRNA-145- 5p expression levels were significantly lower in cancerous tissues (p=0.0024). Conclusion: miRNA-145 expression in ESCC seems to be regulated by hypermethylation of the miRNA-145 promoter region.

AB - Background/Aim: DNA methylation is a common epigenetic change in cancer. However, microRNA (miRNA) regulation by epigenetic alteration, especially CpG island hypermethylation, remains poorly understood in esophageal squamous cell carcinoma (ESCC). Materials and Methods: miRNAs which were up-regulated after de-methylation with 5- aza-2'-deoxycytidine (5-AZA) were analyzed using the Human miFinder 384HC miScript miRNA PCR Array. The DNA methylation level was evaluated by bisulfite-pyrosequencing assay. Results: In two of the cell lines, 20 miRNAs, including miR-145-5p, were found to be up-regulated by more than three-fold after 5-AZA treatment. The miRNA-145 promoter was significantly more hypermethylated in the cancer tissues than in matched normal adjacent esophageal epithelial mucosa (p=0.0042; paired t-test). Moreover, the miRNA-145- 5p expression levels were significantly lower in cancerous tissues (p=0.0024). Conclusion: miRNA-145 expression in ESCC seems to be regulated by hypermethylation of the miRNA-145 promoter region.

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Suppressor microRNA-145 Is epigenetically regulated by promoter hypermethylation in esophageal squamous cell carcinoma

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!!!All Science Journal Classification (ASJC) codes

UN SDG

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