Study on measurement of nanoparticles size based on fluorescence polarization (1st Report) - Development of rotational diffusion coefficient measurement system using fluorescent DNA probe - Development o

Yuki Ishizaki, Terutake Hayashi, Masaki Michihata, Yasuhiro Takaya

研究成果: ジャーナルへの寄稿学術誌査読

抄録

Nanoparticles are attractive materials in the area of nanotechnology, and there is a need to measure the particle size accurately and easily. We suggest a novel particle sizing method based on fluorescence polarization. Particle size evaluation can be achieved from measuring the rotational diffusion coefficient which is sensitive to the particle size. We develop the rotational diffusion coefficient measurement system using fluorescent DNA probe. We verify the feasibility of proposed method by a fundamental experiment. We measure the rotational diffusion coefficient of gold nanoparticle whose diameter is 8.2nm. The rotational diffusion coefficient decreases by 50% compared to the reference value in the measuring range. The result indicates that nanoparticle, whose diameter is as small as 8.2nm, can be measured.

本文言語英語
ページ(範囲)214-219
ページ数6
ジャーナルSeimitsu Kogaku Kaishi/Journal of the Japan Society for Precision Engineering
80
2
DOI
出版ステータス出版済み - 2014

!!!All Science Journal Classification (ASJC) codes

  • 機械工学

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