Studies on the base excision repair (BER) complex in Pyrococcus furiosus

Shinichi Kiyonari, Saki Tahara, Maiko Uchimura, Tsuyoshi Shirai, Sonoko Ishino, Yoshizumi Ishino

研究成果: ジャーナルへの寄稿学術誌査読

16 被引用数 (Scopus)


We have been studying the functions of PCNA (proliferating-cell nuclear antigen) for the assembly and reassembly of the replisome during replication fork progression. We have identified the functional interactions between PCNA and several proteins involved in DNA replication and repair from Pyrococcus furiosus. We recently reported that the activity of UDG (uracil-DNA glycosylase) in P. furiosus (PfuUDG) is stimulated by PCNA (PfuPCNA) in vitro, and identified an atypical PCNA-binding site, AKTLF, in the PfuUDG protein. To understand further the function of the complex in the BER (base excision repair) process, we investigated the AP (apurinic/apyrimidinic) endonuclease, which can process the BER pathway after uracil removal by UDG. Interestingly, one candidate ORF (open reading frame) for the AP endonuclease was found in the operon containing the gene encoding UDG in the P. furiosus genome. However, this ORF did not exhibit any activity. Instead, we identified the AP endonuclease activity from the other candidate gene products, and designated the protein as PfuAP. We discovered a physical interaction between PfuAP and PfuPCNA, suggesting the formation of a BER complex in one of the repair systems in P. furiosus.

ジャーナルBiochemical Society Transactions
出版ステータス出版済み - 2009

!!!All Science Journal Classification (ASJC) codes

  • 生化学


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