Stereoselective synthesis of the head group of archaeal phospholipid PGP-Me to investigate bacteriorhodopsin-lipid interactions

Jin Cui, Satoshi Kawatake, Yuichi Umegawa, Sébastien Lethu, Masaki Yamagami, Shigeru Matsuoka, Fuminori Sato, Nobuaki Matsumori, Michio Murata

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)

抄録

Phosphatidylglycerophosphate methyl ester (PGP-Me), a major constituent of the archaeal purple membrane, is essential for the proper proton-pump activity of bacteriorhodopsin (bR). We carried out the first synthesis of the bisphosphate head group of PGP-Me using H-phosphonate chemistry that led to the production of a simplified PGP-Me analogue with straight alkyl chains. To investigate the role of this head group in the structural and functional integrity of bR, the analogue was used to reconstitute bR into liposomes, in which bR retained the original trimeric structure and light-induced photocycle activity. Enhanced ordering of an alkyl chain of the 2H-labelled analogue was observed in 2H NMR spectra upon interaction with bR. These results together suggest that the bisphosphate moiety plays a role in the proper functioning of bR through the lipid-protein interaction.

本文言語英語
ページ(範囲)10279-10284
ページ数6
ジャーナルOrganic and Biomolecular Chemistry
13
41
DOI
出版ステータス出版済み - 2015

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 物理化学および理論化学
  • 有機化学

フィンガープリント

「Stereoselective synthesis of the head group of archaeal phospholipid PGP-Me to investigate bacteriorhodopsin-lipid interactions」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル