Spheroid formation of hepatocytes using synthetic polymer

Masamichi Kamihira, Keisuke Yamada, Ryuji Hamamoto, Shinji Iijima

研究成果: ジャーナルへの寄稿学術誌査読

31 被引用数 (Scopus)


It is known that hepatocytes show the different morphology depending on the surface condition of cultural substratum. The cells form a floating cell-aggregate called spheroid on proteoglycan-coated or positively charged dishes. The liver cell functions are generally high and maintained for the long-term in the spheroid culture. Since spheroid morphology is preferable for the construction of a bioartificial liver, it is important to develop an effective method of preparing spheroids. In this regard, we examined a preparation method of functional spheroid-like cell-aggregates, in which a synthetic polymer, Eudragit was added to culture medium for inducing liver cell-aggregation. The cell-cell attachment of the aggregate was loose at the beginning of the culture, but it became tight and spheroids were formed 2-3 days after inoculation. When 0.1% Eudragit was added to the medium, the liver functions such as albumin secretion, ammonia removal and urea synthesis were enhanced compared with monolayer and conventional spheroid cultures. The spheroid formation was also performed with suspension culture in a spinner flask. Approximately 80% of the cells inoculated formed spheroids by the addition of the polymer. Moreover, the polymer showed a protective effect from cell damage by agitation. Since this procedure does not require surface for cell attachment, a large amount of spheroids can be prepared in suspension culture.

ジャーナルAnnals of the New York Academy of Sciences
出版ステータス出版済み - 12月 31 1997

!!!All Science Journal Classification (ASJC) codes

  • 神経科学一般
  • 生化学、遺伝学、分子生物学一般
  • 科学史および科学哲学


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