TY - JOUR
T1 - Spatial analysis for exclusive interactions between subgroups I and II of Cucumber mosaic virus in cowpea
AU - Takeshita, Minoru
AU - Shigemune, Naofumi
AU - Kikuhara, Kenji
AU - Furuya, Naruto
AU - Takanami, Yoichi
N1 - Funding Information:
We are especially grateful to Prof. Peter Palukaitis for critical reading of this manuscript. This work was partly supported by Grants-in-Aid for Scientific Research 15028215 and (A)08406004 from The Ministry of Education, Science, Sports and Culture, Japan.
PY - 2004/10/15
Y1 - 2004/10/15
N2 - The dynamics of virus interference in Cucumber mosaic virus (CMV) infection in cowpea were investigated by tissue-blotting and in situ hybridization. Using co-inoculation assays, we discovered that spatial competition between CMV-LE (subgroup I) and CMV-m2 (subgroup II) occurred in the inoculated leaves. Interestingly, competitive interactions between the two viruses also could be observed in the non-inoculated upper leaf tissues of the plants. Furthermore, the pattern of exclusive distribution was observed between challenge and protecting viruses in the serially inoculated leaves. Taken together, it is suggested that the dynamics of competitive interactions between the two subgroups could be characterized by exclusive infection and multiplication of the individual viruses in cowpea plants.
AB - The dynamics of virus interference in Cucumber mosaic virus (CMV) infection in cowpea were investigated by tissue-blotting and in situ hybridization. Using co-inoculation assays, we discovered that spatial competition between CMV-LE (subgroup I) and CMV-m2 (subgroup II) occurred in the inoculated leaves. Interestingly, competitive interactions between the two viruses also could be observed in the non-inoculated upper leaf tissues of the plants. Furthermore, the pattern of exclusive distribution was observed between challenge and protecting viruses in the serially inoculated leaves. Taken together, it is suggested that the dynamics of competitive interactions between the two subgroups could be characterized by exclusive infection and multiplication of the individual viruses in cowpea plants.
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U2 - 10.1016/j.virol.2004.06.046
DO - 10.1016/j.virol.2004.06.046
M3 - Article
C2 - 15380357
AN - SCOPUS:4544385766
SN - 0042-6822
VL - 328
SP - 45
EP - 51
JO - Virology
JF - Virology
IS - 1
ER -