Sp1-like proteins function in the transcription of human ferredoxin genes

Jing Ruey Yeh, Li Chung Hsu, Bon Chu Chung

研究成果: ジャーナルへの寄稿学術誌査読

3 被引用数 (Scopus)

抄録

We characterized a regulatory element located in the-76 to-62 region of the human ferredoxin gene. This region bound to Sp1-like proteins with low affinity, as shown using electrophoretic mobility shift, competition, antibody binding, and Southwestern experiments. The similarity of the regulatory element to Sp1 extends beyond its DNA-binding domain, as cloned Sp1 functioned equally well when fused to a peptide that bound to an irrelevant site. The function of these Sp1-binding sites is mediated through the cAMP-dependent protein kinase (PKA) signaling pathway, because reporter genes downstream of the Sp1-binding sites were not activated in a PKA- deficient cell line. Transfection of the catalytic subunit of PKA restored activated transcription. Similar Sp1-binding sites identified in the CYP11A1 and CYP21 genes also controlled cAMP-dependent transcription of the reporter gene. Our finding of the function of Sp1-like proteins in steroidogenic gene transcription adds one more role Sp1 plays in controlling physiological events. (C) 2000 National Science Council, ROC and S, Karger AG, Basel.

本文言語英語
ページ(範囲)144-151
ページ数8
ジャーナルJournal of Biomedical Science
7
2
DOI
出版ステータス出版済み - 2000
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 内分泌学、糖尿病および代謝内科学
  • 分子生物学
  • 臨床生化学
  • 細胞生物学
  • 生化学、医学
  • 薬理学(医学)

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