Sp1-like proteins function in the transcription of human ferredoxin genes

Jing Ruey Yeh, Li Chung Hsu, Bon Chu Chung

研究成果: ジャーナルへの寄稿学術誌査読

3 被引用数 (Scopus)


We characterized a regulatory element located in the-76 to-62 region of the human ferredoxin gene. This region bound to Sp1-like proteins with low affinity, as shown using electrophoretic mobility shift, competition, antibody binding, and Southwestern experiments. The similarity of the regulatory element to Sp1 extends beyond its DNA-binding domain, as cloned Sp1 functioned equally well when fused to a peptide that bound to an irrelevant site. The function of these Sp1-binding sites is mediated through the cAMP-dependent protein kinase (PKA) signaling pathway, because reporter genes downstream of the Sp1-binding sites were not activated in a PKA- deficient cell line. Transfection of the catalytic subunit of PKA restored activated transcription. Similar Sp1-binding sites identified in the CYP11A1 and CYP21 genes also controlled cAMP-dependent transcription of the reporter gene. Our finding of the function of Sp1-like proteins in steroidogenic gene transcription adds one more role Sp1 plays in controlling physiological events. (C) 2000 National Science Council, ROC and S, Karger AG, Basel.

ジャーナルJournal of Biomedical Science
出版ステータス出版済み - 2000

!!!All Science Journal Classification (ASJC) codes

  • 内分泌学、糖尿病および代謝内科学
  • 分子生物学
  • 臨床生化学
  • 細胞生物学
  • 生化学、医学
  • 薬理学(医学)


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