Solution structure of the N-terminal domain of a replication restart primosome factor, PriC, in Escherichia coli

Takahiko Aramaki, Yoshito Abe, Tsutomu Katayama, Tadashi Ueda

研究成果: ジャーナルへの寄稿学術誌査読

6 被引用数 (Scopus)

抄録

In eubacterial organisms, the oriC-independent primosome plays an essential role in replication restart after the dissociation of the replication DNA-protein complex by DNA damage. PriC is a key protein component in the replication restart primosome. Our recent study suggested that PriC is divided into two domains: an N-terminal and a C-terminal domain. In the present study, we determined the solution structure of the N-terminal domain, whose structure and function have remained unknown until now. The revealed structure was composed of three helices and one extended loop. We also observed chemical shift changes in the heteronuclear NMR spectrum and oligomerization in the presence of ssDNA. These abilities may contribute to the PriC-ssDNA complex, which is important for the replication restart primosome.

本文言語英語
ページ(範囲)1279-1286
ページ数8
ジャーナルProtein Science
22
9
DOI
出版ステータス出版済み - 9月 2013

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

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