Simple and rapid detection of Streptococcus mutans and Streptococcus sobrinus in human saliva by polymerase chain reaction

T. Oho, Y. Yamashita, Y. Shimazaki, M. Kushiyama, Toshihiko Koga

研究成果: ジャーナルへの寄稿学術誌査読

145 被引用数 (Scopus)

抄録

Streptococcus mutans and Streptococcus sobrinus are major pathogens causing dental caries in humans. A simple and rapid method to detect these species in human saliva simultaneously was developed using the polymerase chain reaction (PCR). Chromosomal DNA was extracted by boiling bacterial cells in lysis solution containing 1% Triton X-100. Oligonucleotide primers specific for portions of the glucosyltransferase genes (gtfB of S. mutans and gtfI of S. sobrinus) were designed. After PCR using two sets of these primers, S. mutans and S. sobrinus were specifically identified. The method was capable of amplifying DNA fragments specific for these species from chromosomal DNA extracted from 1 X 103 cells, or from μl of clinical saliva samples containing 1 X 103 colony-forming units of either streptococcal species. A second PCR, using the first PCR product as a template with newly designed internal primers, made it possible to detect 1 x 102 colony-forming units of either streptococcal species in 10 μl of saliva samples. These results indicate that the PCR method developed in this study is useful for detecting S. mutans and S. sobrinus in saliva and that it can be used in epidemiological studies to evaluate the prevalence level of these organisms.

本文言語英語
ページ(範囲)258-262
ページ数5
ジャーナルOral Microbiology and Immunology
15
4
DOI
出版ステータス出版済み - 8月 2000
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 微生物学(医療)
  • 歯学一般
  • 微生物学
  • 免疫学

フィンガープリント

「Simple and rapid detection of Streptococcus mutans and Streptococcus sobrinus in human saliva by polymerase chain reaction」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル