SHARPIN is a novel gene of colorectal cancer that promotes tumor growth potentially via inhibition of p53 expression

Yusuke Nakano, Takaaki Masuda, Takeharu Sakamoto, Noritaka Tanaka, Taro Tobo, Masahiro Hashimoto, Takanari Tatsumi, Hideyuki Saito, Junichi Takahashi, Kensuke Koike, Tadashi Abe, Yuki Ando, Yuki Ozato, Kiyotaka Hosoda, Kosuke Hirose, Satoshi Higuchi, Tomohiko Ikehara, Yuichi Hisamatsu, Takeo Toshima, Yusuke YonemuraTakayuki Ogino, Mamoru Uemura, Hidetoshi Eguchi, Yuichiro Doki, Koshi Mimori

研究成果: ジャーナルへの寄稿学術誌査読

抄録

Colorectal cancer (CRC) is widely prevalent and represents a significant contributor to global cancer‑related mortality. There remains a pressing demand for advance‑ ments in CRC treatment modalities. The E3 ubiquitin ligase is a critical enzyme involved in modulating protein expression levels via posttranslational ubiquitin‑mediated proteolysis, and it is reportedly involved in the progression of various cancers, making it a target of recent interest in anticancer therapy. In the present study, using comprehensive expression analysis involving spatial transcriptomic analysis with single‑cell RNA sequencing in clinical CRC datasets, the ubiquitin‑associated protein Shank‑associated RH domain interactor (SHARPIN) was identified, located on amplified chromosome 8q, which could promote CRC progression. SHARPIN was found to be upregulated in tumor cells, with elevated expression observed in tumor tissues. This heightened expression of SHARPIN was positively associated with lymphatic invasion and served as an independent predictor of a poor prognosis in patients with CRC. In vitro and in vivo analyses using SHARPIN‑overexpressing or ‑knockout CRC cells revealed that SHARPIN overexpression upregulated MDM2, resulting in the downregulation of p53, while SHARPIN silencing or knockout downregulated MDM2, leading to p53 upregulation, which affects cell cycle progression, tumor cell apoptosis and tumor growth in CRC. Furthermore, SHARPIN was found to be overexpressed in several cancer types, exerting significant effects on survival outcomes. In conclusion, SHARPIN represents a newly identified novel gene with the potential to promote tumor growth following apoptosis inhibition and cell cycle progression in part by inhibiting p53 expression via MDM2 upregulation; therefore, SHARPIN represents a potential therapeutic target for CRC.

本文言語英語
論文番号113
ジャーナルInternational journal of oncology
65
6
DOI
出版ステータス出版済み - 12月 2024

!!!All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究

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