Self-assembly of Ni-NTA-modified β-annulus peptides into artificial viral capsids and encapsulation of His-tagged proteins

Kazunori Matsuura, Tomohiro Nakamura, Kenta Watanabe, Takanori Noguchi, Kosuke Minamihata, Noriho Kamiya, Nobuo Kimizuka

研究成果: ジャーナルへの寄稿学術誌査読

33 被引用数 (Scopus)

抄録

β-Annulus peptides bearing Cys at the N-terminal from tomato bushy stunt virus were synthesised using a standard Fmoc-protected solid-phase method, and the peptide was modified with Ni-NTA at the N-terminal. The Ni-NTA-modified β-annulus peptide self-assembled into virus-like nanocapsules of approximately 40 nm in diameter. The critical aggregation concentration of these nanocapsules in 10 mM Tris-HCl buffer (pH 7.3) at 25 °C was 0.053 μM, which is 470 times lower than that of unmodified β-annulus peptides. Moreover, size exclusion chromatography of the peptide assembly indicated encapsulation of His-tagged green fluorescent protein in the Ni-NTA-modified artificial viral capsid.

本文言語英語
ページ(範囲)7869-7874
ページ数6
ジャーナルOrganic and Biomolecular Chemistry
14
33
DOI
出版ステータス出版済み - 2016

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 物理化学および理論化学
  • 有機化学

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