Role of troponin I isoform switching in determining the pH sensitivity of Ca2+ regulation in developing rabbit cardiac muscle

Sachio Morimoto, Takako Goto

研究成果: ジャーナルへの寄稿学術誌査読

25 被引用数 (Scopus)

抄録

Skinned muscle fibers prepared from fetal rabbit heart (28 days of gestation) showed a marked resistance to acidic pH in the Ca2+ regulation of force generation, compared to the fibers prepared from adult heart. SDS-PAGE and immunoblot analysis showed that the slow skeletal troponin I was predominantly expressed in the fetal cardiac muscle, while the cardiac isoform was predominantly expressed in the adult cardiac muscle. Direct exchange of purified slow skeletal and cardiac troponin I isoforms into these skinned muscle fibers revealed that cardiac troponin I made the Ca2+ regulation of contraction sensitive to acidic pH just as in the adult fibers, whereas slow skeletal troponin I made the Ca2+ regulation of contraction resistant to acidic pH just as in the fetal fibers. These results demonstrate that the troponin I isoform switching accounts fully for the change in the pH dependence of Ca2+ regulation of contraction in developmental cardiac muscle. (C) 2000 Academic Press.

本文言語英語
ページ(範囲)912-917
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
267
3
DOI
出版ステータス出版済み - 1月 27 2000

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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