Role of STAT3 in liver regeneration: Survival, DNA synthesis, inflammatory reaction and liver mass recovery

Akira Moh, Yoshiki Iwamoto, Gui Xuan Chai, Samual Shao Min Zhang, Arihiro Kano, Derek D. Yang, Wenjun Zhang, Jun Wang, Joerg J. Jacoby, Bin Gao, Richard A. Flavell, Xin Yuan Fu

研究成果: ジャーナルへの寄稿学術誌査読

118 被引用数 (Scopus)

抄録

The hepatoprotective effect of interleukin-6 (IL-6)/signal transducer and activator of transcription 3 (STAT3) has been well documented. However, reports on the role of IL-6/STAT3 in liver regeneration are conflicting probably due to the fact that the model of Stat3 knockout mice were complicated with obesity and fatty liver, which may cause some secondary effects on liver regeneration. To study the direct role of STAT3 and to circumvent the problems of obesity and fatty liver in liver regeneration, we generated conditional STAT3 knockout in the liver (L-Stat3-/-) using a transthyretin-driven Cre-lox method. The L-Stat3-/- mice were born with the expected Mendelian frequency and showed no obesity or other obvious phenotype. After partial hepatectomy, mortality in the L-Stat3-/- mice was significantly higher than the littermate Stat3f/+ controls in the early time points (<24 h). Hepatocyte DNA synthesis in the survived L-Stat3-/- mice slightly decreased as compared with Stat3f/+ mice at 40 h after partial hepatectomy, whereas similar hepatocyte DNA synthesis was found at other time points and liver mass could be completely recovered in the L-Stat3-/- mice. In another model of liver regeneration induced by subcutaneous injection of carbon tetrachloride (CCl4), hepatocyte DNA synthesis in the CCl4-treated L-Stat3-/- mice also decreased as compared with Stat3f/+ mice at 40 h after injection but not at other time points. In addition, infiltration of neutrophils and monocyte increased in the liver of CCl4-treated L-Stat3-/- mice compared to wild-type mice. In conclusion, STAT3 is required for survival in the acute stage after 70% hepatectomy and plays a role in inflammatory reaction after hepatocyte necrosis. However, the hepatocytic STAT3 may have limited role in liver mass recovery although DNA synthesis may be impaired.

本文言語英語
ページ(範囲)1018-1028
ページ数11
ジャーナルLaboratory Investigation
87
10
DOI
出版ステータス出版済み - 10月 2007
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 分子生物学
  • 細胞生物学

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