Role of Mg2+ ions in flavin recognition by RNA aptamer

Abhigyan Sengupta, Krishna Gavvala, Raj Kumar Koninti, Partha Hazra

研究成果: ジャーナルへの寄稿学術誌査読

10 被引用数 (Scopus)

抄録

The role of Mg2+ ion in flavin (flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN)) recognition by RNA aptamer has been explored through steady state and time-resolved fluorescence, circular dichroism (CD), thermal melting (TM) and isothermal titration calorimetry (ITC) studies. A strong quenching of flavin emission is detected due to stacking interaction with the nucleobases in the mismatched region of aptamer, and it enhances manifold with increasing Mg2+ concentrations. A comparatively lower binding affinity toward FAD compared to FMN is attributed to the presence of intramolecular 'stack' conformer of FAD, which cannot participate in the intermolecular stacking interactions with the nucleobases. CD and TM studies predict that flavin detection causes structural reformation of RNA aptamer. ITC results indicate that flavin detection is thermodynamically feasible and highly enthalpy driven.

本文言語英語
ページ(範囲)240-248
ページ数9
ジャーナルJournal of Photochemistry and Photobiology B: Biology
140
DOI
出版ステータス出版済み - 11月 2014
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 放射線
  • 放射線技術および超音波技術
  • 生物理学
  • 放射線学、核医学およびイメージング

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