Rna-binding protein rbp-p is required for glutelin and prolamine mrna localization in rice endosperm cells[open]

Li Tian, Hong Li Chou, Laining Zhang, Seon Kap Hwang, Shawn R. Starkenburg, Kelly A. Doroshenk, Toshihiro Kumamaru, Thomas W. Okita

研究成果: ジャーナルへの寄稿学術誌査読

43 被引用数 (Scopus)

抄録

In developing rice (Oryza sativa) endosperm, mRNAs of the major storage proteins, glutelin and prolamine, are transported and anchored to distinct subdomains of the cortical endoplasmic reticulum. RNA binding protein RBP-P binds to both glutelin and prolamine mRNAs, suggesting a role in some aspect of their RNA metabolism. Here, we show that rice lines expressing mutant RBP-P mislocalize both glutelin and prolamine mRNAs. Different mutant RBP-P proteins exhibited varying degrees of reduced RNA binding and/or protein-protein interaction properties, which may account for the mislocalization of storage protein RNAs. In addition, partial loss of RBP-P function conferred a broad phenotypic variation ranging from dwarfism, chlorophyll deficiency, and sterility to late flowering and low spikelet fertility. Transcriptome analysis highlighted the essential role of RBP-P in regulating storage protein genes and several essential biological processes during grain development. Overall, our data demonstrate the significant roles of RBP-P in glutelin and prolamine mRNA localization and in the regulation of genes important for plant growth and development through its RNA binding activity and cooperative regulation with interacting proteins.

本文言語英語
ページ(範囲)2529-2552
ページ数24
ジャーナルPlant Cell
30
10
DOI
出版ステータス出版済み - 2018

!!!All Science Journal Classification (ASJC) codes

  • 植物科学
  • 細胞生物学

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