Oxygen plasma that is a kind of external biological stimulus activates the macrophage-like cell. Active oxygen species in the oxygen plasma-irradiated macrophage-like cells in culture medium to investigate the effects of different treatment periods and applied voltages. Macrophage-like cells were activated and increased 10% after 48 h from the short period plasma irradiation of 30 s. The long period irradiation for 60 and 120 s decreased the cell number by almost 33%. The phagocytosis capability increased by 1.5 times during the shorter plasma irradiation period, which would be owing to the increase of active oxygen species inside the macrophage cells.
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