Reconstitution of mouse oogenesis in a dish from pluripotent stem cells

Katsuhiko Hayashi, Orie Hikabe, Yayoi Obata, Yuji Hirao

研究成果: ジャーナルへの寄稿学術誌査読

53 被引用数 (Scopus)

抄録

Generation of functional oocytes in culture from pluripotent stem cells should provide a useful model system for improving our understanding of the basic mechanisms underlying oogenesis. In addition, it has potential applications as an alternative source of oocytes for reproduction. Using the most advanced mouse model in regard to reproductive engineering and stem cell biology, we previously developed a culture method that produces functional primorial germ cells starting from pluripotent cells in culture and described it in a previous protocol. This Protocol Extension describes an adaptation of this existing Protocol in which oogenesis also occurs in vitro, thus substantially modifying the technique. Oocytes generated from embryonic stem cells (ESCs) or induced pluripotent stem cells give rise to healthy pups. Here, we describe the protocol for oocyte generation in culture. The protocol is mainly composed of three different culture stages: in vitro differentiation (IVDi), in vitro growth (IVG), and in vitro maturation (IVM), which in total take ∼5 weeks. In each culture period, there are several checkpoints that enable the number of oocytes being produced in the culture to be monitored. The basic structure of the culture system should provide a useful tool for clarifying the complicated sequence of oogenesis in mammals.

本文言語英語
ページ(範囲)1733-1744
ページ数12
ジャーナルNature Protocols
12
9
DOI
出版ステータス出版済み - 9月 1 2017

!!!All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学一般

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