TY - JOUR
T1 - Real-time fluorescence monitoring of GSK3β-catalyzed phosphoryation by use of a BODIPY-based Zn(II)-Dpa chemosensor
AU - Sakamoto, Takashi
AU - Inoue, Masa aki
AU - Ojida, Akio
AU - Hamachi, Itaru
N1 - Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2009/8/1
Y1 - 2009/8/1
N2 - We developed a new fluorescence assay system for GSK3β-catalyzed kinase reaction using the BODIPY-based fluorescent chemosensor. This system exploits the selective sensing property of the chemosensor for a (i, i+4) bis-phosphorylated peptide, which allows us to conveniently detect the phosphorylation reaction with a fluorescence increase in a real-time fashion.
AB - We developed a new fluorescence assay system for GSK3β-catalyzed kinase reaction using the BODIPY-based fluorescent chemosensor. This system exploits the selective sensing property of the chemosensor for a (i, i+4) bis-phosphorylated peptide, which allows us to conveniently detect the phosphorylation reaction with a fluorescence increase in a real-time fashion.
UR - http://www.scopus.com/inward/record.url?scp=67649909791&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67649909791&partnerID=8YFLogxK
U2 - 10.1016/j.bmcl.2009.05.121
DO - 10.1016/j.bmcl.2009.05.121
M3 - Article
C2 - 19546004
AN - SCOPUS:67649909791
SN - 0960-894X
VL - 19
SP - 4175
EP - 4177
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 15
ER -