Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy

M. Nakano, Z. Ding, H. Kasahara, J. Suehiro

研究成果: 書籍/レポート タイプへの寄稿会議への寄与

3 被引用数 (Scopus)

抄録

This paper reports a new method to determine the size of DNA amplified by polymerase chain reaction (PCR). After PCR, the amplified DNA was attached on dielectric microbeads, then, the DNA labeled microbeads were trapped on a microelectrode by dielectrophoresis (DEP). The size of the DNA was determined by measuring frequency dependent impedance of the trapped microbeads as an impedance spectroscopy. The size of various DNAs, 142, 204, 391, 796 bp, were successfully identified from the impedance spectrograms. This method determines DNA size in 20 min after PCR.

本文言語英語
ホスト出版物のタイトル2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
出版社Institute of Electrical and Electronics Engineers Inc.
ページ1530-1532
ページ数3
ISBN(電子版)9781479989553
DOI
出版ステータス出版済み - 8月 5 2015
イベント18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, 米国
継続期間: 6月 21 20156月 25 2015

出版物シリーズ

名前2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

その他

その他18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
国/地域米国
CityAnchorage
Period6/21/156/25/15

!!!All Science Journal Classification (ASJC) codes

  • 器械工学
  • 電子工学および電気工学

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