TY - JOUR
T1 - Rapid enzyme-linked immunosorbent assay with functional magnetite particles
AU - Shinkai, Masashige
AU - Wang, Jin
AU - Kamihira, Masamichi
AU - Iwata, Masashi
AU - Honda, Hiroyuki
AU - Kobayashi, Takeshi
PY - 1992
Y1 - 1992
N2 - Ultrafine magnetite particles were applied to enzyme-linked immunosorbent assay (ELISA). For the assay of mouse IgG, anti-mouse IgG antibody or protein A was immobilized on the magnetite particles. The assay strategy was based on the sandwich method. Using anti-mouse IgG-magnetite and the spectrofluorometry, the mouse IgG standard curve was linear when the IgG concentration was between 5 μg/l-5 mg/l. Using these functional magnetite particles, we could shorten the assay time to one thirty-fifth that of the conventional method.
AB - Ultrafine magnetite particles were applied to enzyme-linked immunosorbent assay (ELISA). For the assay of mouse IgG, anti-mouse IgG antibody or protein A was immobilized on the magnetite particles. The assay strategy was based on the sandwich method. Using anti-mouse IgG-magnetite and the spectrofluorometry, the mouse IgG standard curve was linear when the IgG concentration was between 5 μg/l-5 mg/l. Using these functional magnetite particles, we could shorten the assay time to one thirty-fifth that of the conventional method.
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U2 - 10.1016/0922-338X(92)90683-L
DO - 10.1016/0922-338X(92)90683-L
M3 - Article
AN - SCOPUS:0026545046
SN - 0922-338X
VL - 73
SP - 166
EP - 168
JO - Journal of Fermentation and Bioengineering
JF - Journal of Fermentation and Bioengineering
IS - 2
ER -