@inbook{4c152b49bb2e447e8569274021d80b7c,
title = "Quantitatively Controlled Intercellular Mitochondrial Transfer by Cell Fusion-Based Method Using a Microfluidic Device",
abstract = "Quantitative control of mitochondrial transfer is a promising approach for genetic manipulation of mitochondrial DNA (mtDNA) because it enables precise modulation of heteroplasmy. Furthermore, single mitochondrion transfer from a mtDNA mutation-accumulated cell to a mtDNA-less (ρ0) cell potentially achieves homoplasmy of mutated mtDNA. Here we describe the method for quantitative control of mitochondrial transfer including achieving single mitochondrion transfer between live single cells using a microfluidic device.",
author = "Wada, {Ken Ichi} and Kazuo Hosokawa and Yoshihiro Ito and Mizuo Maeda",
note = "Funding Information: This work was supported by Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research (KAKENHI) (Grant Numbers 22220009, 26650068 and 16 K07207), Center of Innovation Program from Japan Science and Technology Agency, and Single Cell Project from RIKEN. Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1270-5_3",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "39--47",
booktitle = "Methods in Molecular Biology",
}