TY - JOUR
T1 - Quantitative modeling of regular retinal microglia distribution
AU - Endo, Yoshie
AU - Asanuma, Daisuke
AU - Namiki, Shigeyuki
AU - Sugihara, Kei
AU - Hirose, Kenzo
AU - Uemura, Akiyoshi
AU - Kubota, Yoshiaki
AU - Miura, Takashi
N1 - Funding Information:
This work is financially supported by JSPS KAKENHI (Grant Nos. 20H03427 to Kenzo Hirose, and 20H02875 to Daisuke Asanuma). The authors would like to thank Dr Shuji Ishihara (University of Tokyo) for discussions and comments, and Dr Tristan Qingyun Li (Washington University School of Medicine) for providing clustering data of scRNASeq, and Enago (www.enago.jp) for the English language review.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Microglia are resident immune cells in the central nervous system, showing a regular distribution. Advancing microscopy and image processing techniques have contributed to elucidating microglia’s morphology, dynamics, and distribution. However, the mechanism underlying the regular distribution of microglia remains to be elucidated. First, we quantitatively confirmed the regularity of the distribution pattern of microglial soma in the retina. Second, we formulated a mathematical model that includes factors that may influence regular distribution. Next, we experimentally quantified the model parameters (cell movement, process formation, and ATP dynamics). The resulting model simulation from the measured parameters showed that direct cell–cell contact is most important in generating regular cell spacing. Finally, we tried to specify the molecular pathway responsible for the repulsion between neighboring microglia.
AB - Microglia are resident immune cells in the central nervous system, showing a regular distribution. Advancing microscopy and image processing techniques have contributed to elucidating microglia’s morphology, dynamics, and distribution. However, the mechanism underlying the regular distribution of microglia remains to be elucidated. First, we quantitatively confirmed the regularity of the distribution pattern of microglial soma in the retina. Second, we formulated a mathematical model that includes factors that may influence regular distribution. Next, we experimentally quantified the model parameters (cell movement, process formation, and ATP dynamics). The resulting model simulation from the measured parameters showed that direct cell–cell contact is most important in generating regular cell spacing. Finally, we tried to specify the molecular pathway responsible for the repulsion between neighboring microglia.
UR - https://www.scopus.com/pages/publications/85119703760
UR - https://www.scopus.com/pages/publications/85119703760#tab=citedBy
U2 - 10.1038/s41598-021-01820-3
DO - 10.1038/s41598-021-01820-3
M3 - Article
C2 - 34811401
AN - SCOPUS:85119703760
SN - 2045-2322
VL - 11
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 22671
ER -