@inbook{387024e7fbae49ba9d3d016b7e6ff9bf,
title = "Quantitative Lipidomics of Biological Samples Using Supercritical Fluid Chromatography Mass Spectrometry",
abstract = "Lipidomics has attracted attention in the discovery of unknown biomolecules and for capturing the changes in metabolism caused by genetic and environmental factors in an unbiased manner. However, obtaining reliable lipidomics data, including structural diversity and quantification data, is still challenging. Supercritical fluid chromatography (SFC) is a suitable technique for separating lipid molecules with high throughput and separation efficiency. Here, we describe a quantitative lipidomics method using SFC coupled with mass spectrometry. This technique is suitable for characterizing the structural diversity of lipids (e.g., phospholipids, sphingolipids, glycolipids, and glycerolipids) with high quantitative accuracy to understand their biological functions.",
keywords = "Cells, Extracellular vesicles, Glycerolipids, Glycolipids, Lipidomics, Lipoproteins, Liquid-liquid extraction, Mass spectrometry, Organs, Phospholipids, Plasma, Quantitative analysis, Sphingolipids, Supercritical fluid chromatography, Tissues",
author = "Hiroaki Takeda and Yoshihiro Izumi and Takeshi Bamba",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.",
year = "2025",
doi = "10.1007/978-1-0716-4334-1_7",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "131--152",
booktitle = "Methods in Molecular Biology",
}