Quantitative image analysis of nuclear chromatin distribution for cytological diagnosis

Ryotaro Jingu, Masafumi Ohki, Sumiko Watanabe, Sadafumi Tamiya, Setsuo Sugishima, Tsunehisa Kaku

研究成果: ジャーナルへの寄稿学術誌査読

6 被引用数 (Scopus)


Objective: We previously reported on the classification of the nuclear chromatin distribution into 3 types, that is peripheral (P), mixed (M) and central (C), which were related to the malignancy of cervical glandular lesions. However, the classification was subjective as it was performed by visual assessment. In the present study, quantitative assessment of nuclear chromatin distribution using image analysis by computer was applied for objective classification of cervical squamous epithelial lesions, which are the most common cervical lesions. Study Design: A total of 130 cells (44 cells of type P, 78 of type M and 8 of type C) from cytological specimens of cervical squamous epithelial lesions (dysplasia, squamous cell carcinoma) were analyzed. An image analysis program was developed as a plug-in macro program of an existing image processing software. The radial distribution (RD) value, which represents the gradient of the staining intensity from the center to the edge of a nucleus, was defined as an index of the chromatin distribution. Results: The RD values calculated in type P, type M and type C cells showed significant statistical differences as assessed by the t test (p < 0.001). Conclusions: Quantification of the nuclear chromatin distribution by image analysis is fast and highly objective. The RD value could be useful as an index for malignancy.

ジャーナルActa Cytologica
出版ステータス出版済み - 10月 2011

!!!All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 組織学


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