TY - JOUR
T1 - Protocol to generate xenogeneic-free/serum-free human dental pulp stem cells
AU - Sonoda, Soichiro
AU - Yamaza, Haruyoshi
AU - Yoshimaru, Koichiro
AU - Taguchi, Tomoaki
AU - Yamaza, Takayoshi
N1 - Funding Information:
We thank all the authors of the original study ( Iwanaka et al., 2020 ) from which this protocol was generated. This work was supported by the grants-in-aid for Early-Career Scientists (JSPS KAKENHI grant number JP19K18945 and JP21K16932 to S.S.) of Japan Society for the Promotion of Science (JSPS).
Publisher Copyright:
© 2022
PY - 2022/6/17
Y1 - 2022/6/17
N2 - Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinical-grade products under a xenogeneic-free/serum-free condition. We also demonstrate the characterization of hDPSCs by CFU-F, flow cytometric, and in vitro multipotent assays. For complete details on the use and execution of this protocol, please refer to Iwanaka et al. (2020).
AB - Human dental pulp stem cell (hDPSCs)-based therapy is a feasible option for regenerative medicine, such as dental pulp regeneration. Here, we show the steps needed to colony-forming unit-fibroblasts (CFU-F)-based isolation, expansion, and cryopreservation of hDPSCs for manufacturing clinical-grade products under a xenogeneic-free/serum-free condition. We also demonstrate the characterization of hDPSCs by CFU-F, flow cytometric, and in vitro multipotent assays. For complete details on the use and execution of this protocol, please refer to Iwanaka et al. (2020).
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U2 - 10.1016/j.xpro.2022.101386
DO - 10.1016/j.xpro.2022.101386
M3 - Article
C2 - 35592060
AN - SCOPUS:85129917495
SN - 2666-1667
VL - 3
JO - STAR Protocols
JF - STAR Protocols
IS - 2
M1 - 101386
ER -
