Proteomic and metabolomic analyses of the white-rot fungus phanerochaete chrysosporium exposed to exogenous benzoic acid

Fumiko Matsuzaki, Motoyuki Shimizu, Hiroyuki Wariishi

研究成果: ジャーナルへの寄稿学術誌査読

85 被引用数 (Scopus)

抄録

Intracellular processes of the white-rot basidiomycete Phanerochaete chrysosporium involved in the metabolism of benzoic acid (BA) were investigated at the proteome and metabolome level. Up-regulation of aryl-alcohol dehydrogenase, arylaldehyde dehydrogenase, and cytochrome P450s was observed upon addition of exogenous BA, suggesting that these enzymes play key roles in its metabolism. Intracellular metabolic shifts from the short-cut TCA/glyoxylate bicycle system to the TCA cycle and an increased flux in the TCA cycle indicated activation of the heme biosynthetic pathway and the production of NAD(P)H. In addition, combined analyses of proteome and metabolome clearly indicated the role of trehalose as a storage disaccharide and that the mannitol cycle plays a role in an alternative energy-producing pathway.

本文言語英語
ページ(範囲)2342-2350
ページ数9
ジャーナルJournal of Proteome Research
7
6
DOI
出版ステータス出版済み - 6月 2008

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 化学一般

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