Production of Monoclonal Antibodies Against Leptospiral Genus-Specific Protein Antigen and Localization of the Antigen by Immunoelectron Microscopy

Noritsugu Sakamoto, Etsuro Ono, Hiroshi Kida, Ryo Yanagawa

研究成果: ジャーナルへの寄稿学術誌査読

10 被引用数 (Scopus)

抄録

Monoclonal antibodies were first produced against a leptospiral genus-specific protein antigen (GP-Ag) isolated from Leptospira interrogans serovar kremastos strain Kyoto. The representative monoclonal antibody (GP-7) did not agglutinate kremastos Kyoto but reacted with Triton X-100 treated kreamastos Kyoto, andamana, patoc and Urawa in enzyme-linked immunosorbent assay (ELISA), indicating that the monoclonal antibody recognized the genus-specific determinant of GP-Ag. The localization of GP-Ag was investigated by immunoelectron microscopy with the iodinated antibody and peroxidase-labeled antibody. The genus-specific determinant of GP-Ag recognized by GP-7 was located on the subsurface of the leptospiral envelope.

本文言語英語
ページ(範囲)557-563
ページ数7
ジャーナルZentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. A
259
4
DOI
出版ステータス出版済み - 1985
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 免疫学

フィンガープリント

「Production of Monoclonal Antibodies Against Leptospiral Genus-Specific Protein Antigen and Localization of the Antigen by Immunoelectron Microscopy」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル