Processing-independent In Vitro translocation of cytochrome P-450(SCC) precursor across mitochondrial membranes

Weijia Ou, Ken Ichirou Morohashi, Yoshiaki Fujii-kuriyama, Tsuneo Omura

研究成果: ジャーナルへの寄稿学術誌査読

42 被引用数 (Scopus)

抄録

In the presence of a membrane-permeable metal chelator, bovine adrenal cortex mitochondria imported P-450(SCC) precursor without processing of the aminoterminal extension peptide. The imported precursor was bound to the matrix side surface of the inner membrane. When the inhibition due to the metal chelator was removed, the imported precursor was processed to the mature form. Unprocessed precursor was also detected in mitochondria when the import reaction was carried out at relatively low temperature. These results suggest that the translocation of P-450(SCC) precursor across mitochondrial membranes is independent of its processing to the mature form. Both membrane-bound and solubilized P-450(SCC) could be cleaved by trypsin into two fragments with molecular weights of 29 kDa and 26 kDa, respectively, suggesting a two-domain structure of the molecule. The in vitro-imported and processed P-450(SCC) was also cleaved by trypsin in the same way. This finding indicated that the in vitro-imported and processed P-450(SCC) has the same conformation as the native form.

本文言語英語
ページ(範囲)1287-1296
ページ数10
ジャーナルJournal of biochemistry
100
5
DOI
出版ステータス出版済み - 10月 1986
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

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