Preparation of lactose-modified cellulose films by a nonaqueous enzymatic reaction and their biofunctional characteristics as a scaffold for cell culture

Kei Esaki, Shingo Yokota, Shizuka Egusa, Yuri Okutani, Yukiko Ogawa, Takuya Kitaoka, Masahiro Goto, Hiroyuki Wariishi

研究成果: ジャーナルへの寄稿学術誌査読

27 被引用数 (Scopus)

抄録

Enzymatic glyco-modification of transparent cellulose films with lactose was achieved by nonaqueous biocatalysis, and rat hepatocyte attachment behavior to the lactose-modified cellulose films was investigated. Regenerated cellulose films were incubated with lactose using a surfactant-enveloped cellulase in lithium chloride/dimethylacetamide solvent at 37°C for 24 h, and lactose molecules were successfully introduced to the cellulose films. The initial cell adhesion on the lactose-modified cellulose films was superior to the original cellulose film. In the absence of serum, hepatocytes were significantly attached only on the lactose-modified cellulose films. This process was markedly suppressed by the addition of free lactose as an inhibitor. These results suggest that such cell adhesion proceeded through a direct interaction between galactose residues on the cellulose films and asialoglycoprotein receptors on the rat liver cell surface. This novel approach for surface glyco-modification of a cellulose matrix and its biofunctional properties are expected to provide potential application as a bioactive scaffold for cell culture engineering.

本文言語英語
ページ(範囲)1265-1269
ページ数5
ジャーナルBiomacromolecules
10
5
DOI
出版ステータス出版済み - 5月 11 2009

!!!All Science Journal Classification (ASJC) codes

  • バイオエンジニアリング
  • 生体材料
  • ポリマーおよびプラスチック
  • 材料化学

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