Preparation of anti-2,4-dichlorophenol and 2,4-dichlorophenoxyacetic acid monoclonal antibodies

Hiroyuki Tanaka, Shayu Yan, Norio Miura, Yukihiro Shoyama

研究成果: ジャーナルへの寄稿学術誌査読

14 被引用数 (Scopus)

抄録

The ratios of hapten and bovine serum albumin (BSA) in an antigen conjugate were determined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Hybridomas secreting monoclonal antibodies against 2,4-dichlorophenoxyacetic acid (2,4-D) were produced by fusing 2,4-D-BSA conjugate-immunized splenocytes with a HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A substantial cross-reaction was observed for 2,4-dichlorophenol (2,4-DP) when compared with that observed for 2,4-D. The full measurement range for this assay is 0.2-3 μg ml-1 for 2,4-DP. On the other hand, the range for 2,4-D is between 1 and 20 μg ml -1.

本文言語英語
ページ(範囲)101-107
ページ数7
ジャーナルCytotechnology
42
2
DOI
出版ステータス出版済み - 2003

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生体医工学
  • 臨床生化学
  • 細胞生物学

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