Phylogenetic relationships among tea cultivars based on AFLP analysis

Seonha Lee, Jungho Kim, Junichi Sano, Yukio Ozaki, Hiroshi Okubo

研究成果: ジャーナルへの寄稿学術誌査読

8 被引用数 (Scopus)


Several modifications of the AFLP protocol were made to simplify the analysis, i.e., the amount of the reagents for restriction digestion, ligation of adaptors, preamplification and selective amplification, the primers without labeling, and the gel staining with ethidium bromide. The phylogenetic relationships among 37 accessions of the genus Camellia were assessed using the modified AFLP technique and cluster analysis. The accessions, 24 Japanese tea cultivars, seven Korean wild tea varieties and six Camellia species closely related to tea, were clustered into five groups; Japanese tea cultivars closely related to 'Yabukita', those not closely related to 'Yabukita', Chinese tea cultivars and Korean wild tea varieties, Assam type black tea cultivars, and Camellia species closely related to tea. The results of this study indicated that the morphological characteristics and the origins of the tea cultivars were well reflected in the clustering of the cultivars based on the AFLP analysis, and that the AFLP technique might find its use as an efficient and effective tool for determining phylogenetic relationships among tea cultivars.

ジャーナルJournal of the Faculty of Agriculture, Kyushu University
出版ステータス出版済み - 2月 2003

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 農業および作物学


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