Partial impairment of interleukin-12 (IL-12) and IL-18 signaling in Tyk2-deficient mice

Kazuya Shimoda, Hiroko Tsutsui, Kenichi Aoki, Kouji Kato, Tadashi Matsuda, Akihiko Numata, Ken Takase, Tetsuya Yamamoto, Hideyuki Nukina, Tomoaki Hoshino, Yoshinobu Asano, Hisashi Gondo, Takashi Okamura, Seiichi Okamura, Kei Ichi Nakayama, Kenji Nakanishi, Yoshiyuki Niho, Mine Harada

研究成果: ジャーナルへの寄稿学術誌査読

65 被引用数 (Scopus)

抄録

Tyk2 is activated in response to interleukin-12 (IL-12) and is essential for IL-12-induced T-cell function, including interferon-γ (IFN-γ) production and Th1 cell differentiation. Because IL-12 is a stimulatory factor for natural killer (NK) cell-mediated cytotoxicity, we examined whether tyk2 is required for IL-12-induced NK cell activity. IL-12-induced NK cell activity in cells from tyk2-deficient mice was drastically reduced compared to that in cells from wild-type mice. IL-18 shares its biologic functions with IL-12. However, the molecular mechanism of IL-18 signaling, which activates an IL-1 receptor-associated kinase and nuclear translocation of nuclear factor-KB, is different from that of IL-12. We next examined whether biologic functions induced by IL-18 are affected by the absence of tyk2. NK cell activity and IFN-γ production induced by IL-18 were reduced by the absence of tyk2. Moreover, the synergistic effect of IL-12 and IL-18 for the production of IFN-γ was also abrogated by the absence of tyk2. This was partially due to the absence of any up-regulation of the IL-18 receptor treated with IL-12, and it might suggest the presence of the crosstalk between Jak-Stat and mitogen-activated protein kinase pathways in cytokine signaling.

本文言語英語
ページ(範囲)2094-2099
ページ数6
ジャーナルBlood
99
6
DOI
出版ステータス出版済み - 3月 15 2002

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 免疫学
  • 血液学
  • 細胞生物学

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