TY - JOUR
T1 - Oxygen-binding Protein Fiber and Microgel
T2 - Supramolecular Myoglobin-Poly(acrylate) Conjugates
AU - Ono, Toshikazu
AU - Hisaoka, Yasushi
AU - Onoda, Akira
AU - Oohora, Koji
AU - Hayashi, Takashi
N1 - Funding Information:
This work was supported by Grants-in-Aid for Scientific Research (Innovative Areas "Element Block", area 2401, KAKENHI 25102527 and 25102534) from MEXT, the Japan Society for the Promotion of Science (JSPS), and JST, Strategic International Collaborative Research Program, SICORP. A.O. acknowledges a support from Japan Association of Chemical Innovation (JACI). T.O. acknowledges a support from Research Fellowship of JSPS. T.H. acknowledges a support from the Sekisui Chemical Grant Program.
Publisher Copyright:
© 2016 Wiley-VCH Verlag GmbH & Co. KGaA.
PY - 2016/4/5
Y1 - 2016/4/5
N2 - A supramolecular conjugate of myoglobin (Mb) and water-soluble poly(acrylate), (PA5k and PA25k, where 5k and 25k represent the molecular weight of the polymers, respectively), is constructed on the basis of a noncovalent heme-heme pocket interaction. The modified heme with an amino group linked to the terminus of one of the heme-propionates is coupled to the side-chain carboxyl groups of poly(acrylate) activated by N-hydroxysuccinimide. The ratios of the heme-modified monomer unit and the unmodified monomer unit (m:n) in the polymer chains of Heme-PA5k and Heme-PA25k were determined to be 4.5:95.5 and 3.1:96.9, respectively. Subsequent addition of apoMb to the conjugated polymers provides Mb-connected fibrous nanostructures confirmed by atomic force microscopy. A mixture of the heme-modified polymer and dimeric apomyoglobin as a cross-linker forms a microgel in which the reconstituted myoglobin retains its native exogenous ligand binding activity.
AB - A supramolecular conjugate of myoglobin (Mb) and water-soluble poly(acrylate), (PA5k and PA25k, where 5k and 25k represent the molecular weight of the polymers, respectively), is constructed on the basis of a noncovalent heme-heme pocket interaction. The modified heme with an amino group linked to the terminus of one of the heme-propionates is coupled to the side-chain carboxyl groups of poly(acrylate) activated by N-hydroxysuccinimide. The ratios of the heme-modified monomer unit and the unmodified monomer unit (m:n) in the polymer chains of Heme-PA5k and Heme-PA25k were determined to be 4.5:95.5 and 3.1:96.9, respectively. Subsequent addition of apoMb to the conjugated polymers provides Mb-connected fibrous nanostructures confirmed by atomic force microscopy. A mixture of the heme-modified polymer and dimeric apomyoglobin as a cross-linker forms a microgel in which the reconstituted myoglobin retains its native exogenous ligand binding activity.
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U2 - 10.1002/asia.201501415
DO - 10.1002/asia.201501415
M3 - Article
C2 - 26890010
AN - SCOPUS:84976240402
SN - 1861-4728
VL - 11
SP - 1036
EP - 1042
JO - Chemistry - An Asian Journal
JF - Chemistry - An Asian Journal
IS - 7
ER -