Oxygen-binding Protein Fiber and Microgel: Supramolecular Myoglobin-Poly(acrylate) Conjugates

Toshikazu Ono, Yasushi Hisaoka, Akira Onoda, Koji Oohora, Takashi Hayashi

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)


A supramolecular conjugate of myoglobin (Mb) and water-soluble poly(acrylate), (PA5k and PA25k, where 5k and 25k represent the molecular weight of the polymers, respectively), is constructed on the basis of a noncovalent heme-heme pocket interaction. The modified heme with an amino group linked to the terminus of one of the heme-propionates is coupled to the side-chain carboxyl groups of poly(acrylate) activated by N-hydroxysuccinimide. The ratios of the heme-modified monomer unit and the unmodified monomer unit (m:n) in the polymer chains of Heme-PA5k and Heme-PA25k were determined to be 4.5:95.5 and 3.1:96.9, respectively. Subsequent addition of apoMb to the conjugated polymers provides Mb-connected fibrous nanostructures confirmed by atomic force microscopy. A mixture of the heme-modified polymer and dimeric apomyoglobin as a cross-linker forms a microgel in which the reconstituted myoglobin retains its native exogenous ligand binding activity.

ジャーナルChemistry - An Asian Journal
出版ステータス出版済み - 4月 5 2016

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 有機化学


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