Optical characterization of the antigen-antibody thin layer using the whispering gallery mode

Takeshi Tajiri, Shuzo Matsumoto, Toshihiko Imato, Toshihiro Okamoto, Masanobu Haraguchi

    研究成果: ジャーナルへの寄稿学術誌査読

    2 被引用数 (Scopus)

    抄録

    We immobilized an antibody (anti-β-Galactosidase) on a polystyrene microsphere by using a covalent bond, and observed the resonance peaks in the scattered light intensity spectra related to the whispering gallery mode (WGM) excitation of the microsphere. The amount and the optical parameters, i.e., thickness and refractive index, of anti-β-Galactosidase on the sphere surface were evaluated based on an absorbance measurement and a resonance peak shift measurement, respectively. Moreover, we measured the variation of the WGM spectra depending on the concentration of the enzyme solution (β-Galactosidase), which allowed us to optically evaluate the thickness and the refractive index of the antigen-antibody layer from the shift of the WGM spectra peak.

    本文言語英語
    ページ(範囲)799-804
    ページ数6
    ジャーナルanalytical sciences
    30
    8
    DOI
    出版ステータス出版済み - 2014

    !!!All Science Journal Classification (ASJC) codes

    • 分析化学

    フィンガープリント

    「Optical characterization of the antigen-antibody thin layer using the whispering gallery mode」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル