Nucleation-synchronized strand displacement for highly sensitive DNA analysis.

Kazuya Hirata; Yuichi Sato; Arihiro Kano; Toshihiro Akaike; Atsushi Maruyama

Nucleation-synchronized strand displacement for highly sensitive DNA analysis.

Kazuya Hirata, Yuichi Sato, Arihiro Kano, Toshihiro Akaike, Atsushi Maruyama

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

抄録

Nucleation process is a rate-limiting step of DNA strand exchange reactions between double helical DNA and its complementary DNA. Here we designed the double stranded probes that have single stranded portion. The single stranded portion is responsible for the nucleation process and mismatch recognition. A single base mismatch in 25mer DNAs could be detected in a few minutes at ambient temperatures. Using this method, various types of SNPs are rapidly and reliably detected without any aids of enzymes or a special instrument.

本文言語	英語
ページ（範囲）	267-268
ページ数	2
ジャーナル	Nucleic acids symposium series (2004)
号	48
出版ステータス	出版済み - 1月 1 2004
外部発表	はい

!!!All Science Journal Classification (ASJC) codes

医学（全般）

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

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引用スタイル

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AU - Maruyama, Atsushi

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Nucleation-synchronized strand displacement for highly sensitive DNA analysis.

抄録

!!!All Science Journal Classification (ASJC) codes

UN SDG

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