TY - JOUR
T1 - Novel localizations and interactions of intercellular bridge proteins revealed by proteomic profiling
AU - Iwamori, Tokuko
AU - Iwamori, Naoki
AU - Matsumoto, Masaki
AU - Imai, Hiroyuki
AU - Ono, Etsuro
N1 - Publisher Copyright:
© 2020 The Author(s) 2020. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved.
PY - 2020/4/24
Y1 - 2020/4/24
N2 - Intercellular bridges (ICBs) connecting germ cells are essential for spermatogenesis, and their deletion causes male infertility. However, the functions and component factors of ICBs are still unknown. We previously identified novel ICB-associated proteins by proteomics analysis using ICB enrichment. Here, we performed immunoprecipitation-proteomics analyses using antibodies specific to known ICB proteins MKLP1, RBM44, and ectoplasmic specialization-associated protein KIAA1210 and predicted protein complexes in the ICB cores. KIAA1210, its binding protein topoisomerase2B (TOP2B), and tight junction protein ZO1 were identified as novel ICB proteins. On the other hand, as well as KIAA1210 and TOP2B, MKLP1 and RBM44, but not TEX14, were localized at the XY body of spermatocytes, suggesting that there is a relationship between ICB proteins and meiotic chromosomes. Moreover, small RNAs interacted with an ICB protein complex that included KIAA1210, RBM44, and MKLP1. These results indicate dynamic movements of ICB proteins and suggest that ICB proteins could be involved not only in the communication between germ cells but also in their epigenetic regulation. Our results provide a novel perspective on the function of ICBs and could be helpful in revealing the biological function of the ICB.
AB - Intercellular bridges (ICBs) connecting germ cells are essential for spermatogenesis, and their deletion causes male infertility. However, the functions and component factors of ICBs are still unknown. We previously identified novel ICB-associated proteins by proteomics analysis using ICB enrichment. Here, we performed immunoprecipitation-proteomics analyses using antibodies specific to known ICB proteins MKLP1, RBM44, and ectoplasmic specialization-associated protein KIAA1210 and predicted protein complexes in the ICB cores. KIAA1210, its binding protein topoisomerase2B (TOP2B), and tight junction protein ZO1 were identified as novel ICB proteins. On the other hand, as well as KIAA1210 and TOP2B, MKLP1 and RBM44, but not TEX14, were localized at the XY body of spermatocytes, suggesting that there is a relationship between ICB proteins and meiotic chromosomes. Moreover, small RNAs interacted with an ICB protein complex that included KIAA1210, RBM44, and MKLP1. These results indicate dynamic movements of ICB proteins and suggest that ICB proteins could be involved not only in the communication between germ cells but also in their epigenetic regulation. Our results provide a novel perspective on the function of ICBs and could be helpful in revealing the biological function of the ICB.
KW - KIAA1210
KW - MKLP1
KW - RBM44
KW - TEX14
KW - ZO1
KW - ectoplasmic specialization
KW - intercellular bridge
KW - spermatogenesis
KW - testis
KW - topoisomerase II beta
UR - http://www.scopus.com/inward/record.url?scp=85084167538&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85084167538&partnerID=8YFLogxK
U2 - 10.1093/biolre/ioaa017
DO - 10.1093/biolre/ioaa017
M3 - Article
C2 - 31995159
AN - SCOPUS:85084167538
SN - 0006-3363
VL - 102
SP - 1134
EP - 1144
JO - Biology of reproduction
JF - Biology of reproduction
IS - 5
ER -