TY - GEN
T1 - Nanowire lysis and DNA extracrtion from a single bacterium for bacteria analysis
AU - Yasui, Takao
AU - Otsuka, Kohei
AU - Takeuchi, Masaki
AU - Yanagida, Takeshi
AU - Kaji, Noritada
AU - Kanai, Masaki
AU - Rahong, Sakon
AU - Nagashima, Kazuki
AU - Naito, Toyohiro
AU - Kawai, Tomoji
AU - Baba, Yoshinobu
N1 - Funding Information:
This work was partly supported by the ImPACT Program of the Council for Science, Technology and Innovation (Cabinet Office, Government of Japan), the Nanotechnology Platform Program (Molecule and Material Synthesis) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), the JSPS Grant-in-Aid for Scientific Research (A) 24241050, and a Grant-in-Aid for Scientific Research on Innovative Areas "Innovative Materials Engineering Based on Biological Diversity" 15H01599 from MEXT, Japan.
Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - Exploiting the method to extract DNA molecules from bacteria makes it possible to analyze bacteria in a short time. Here, we realized DNA extraction from a single bacterium via physical interactions between nanowires and bacteria; nanowire lysis. The combination of nanowire lysis and heating at 94°C could help us to extract genome DNA from bacteria, efficiently.
AB - Exploiting the method to extract DNA molecules from bacteria makes it possible to analyze bacteria in a short time. Here, we realized DNA extraction from a single bacterium via physical interactions between nanowires and bacteria; nanowire lysis. The combination of nanowire lysis and heating at 94°C could help us to extract genome DNA from bacteria, efficiently.
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UR - http://www.scopus.com/inward/citedby.url?scp=84983359506&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84983359506
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 320
EP - 322
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -