TY - JOUR
T1 - Nanostructure Control of an Antibiotic-Based Polyion Complex Using a Series of Polycations with Different Side-Chain Modification Rates
AU - Ahmad, Asmariah
AU - Nii, Teruki
AU - Mori, Takeshi
AU - Katayama, Yoshiki
AU - Toyofuku, Masanori
AU - Kishimura, Akihiro
N1 - Funding Information:
This research was supported in part by JSPS KAKENHI (Grant Nos. JP18H03534 and JP22H02202), a research grant from the Iketani Science and Technology Foundation (No. 0321035‐A), and a Monbukagakusho Scholarship, MEXT (to A.A.). The authors are grateful to the Nanotechnology Platform Project (Molecules and Materials Synthesis, and Advanced Characterization Nanotechnology Platform) of MEXT for their valuable support in TEM analysis.
Publisher Copyright:
© 2022 Wiley-VCH GmbH.
PY - 2022/10
Y1 - 2022/10
N2 - Developing nanovehicles for delivering antibiotics is a promising approach to overcome the issue of antibiotic resistance. This study aims to utilize a polyion complex (PICs) system for developing novel nanovehicles for polymyxin-type antibiotics, which are known as last resort drugs. The formation of antibiotic-based PIC nanostructures is investigated using colistimethate sodium (CMS), an anionic cyclic short peptide, and a series of block catiomers bearing different amounts of guanidinium moieties on their side chains. In addition, only the modified catiomer, and not the unmodified catiomer, self-assembles with CMS, implying the importance of the guanidine moieties for enhancing the interaction between the catiomer and CMS via the formation of multivalent hydrogen bonding. Moreover, micellar and vesicular PIC nanostructures are selectively formed depending on the ratio of the guanidine residues. Size-exclusion chromatography reveals that the encapsulation efficiency of CMS is dependent on the guanidinium modification ratio. The antimicrobial activity of the PIC nanostructures is also confirmed, indicating that the complexation of CMS in the PICs and further release from the PICs successfully occurs.
AB - Developing nanovehicles for delivering antibiotics is a promising approach to overcome the issue of antibiotic resistance. This study aims to utilize a polyion complex (PICs) system for developing novel nanovehicles for polymyxin-type antibiotics, which are known as last resort drugs. The formation of antibiotic-based PIC nanostructures is investigated using colistimethate sodium (CMS), an anionic cyclic short peptide, and a series of block catiomers bearing different amounts of guanidinium moieties on their side chains. In addition, only the modified catiomer, and not the unmodified catiomer, self-assembles with CMS, implying the importance of the guanidine moieties for enhancing the interaction between the catiomer and CMS via the formation of multivalent hydrogen bonding. Moreover, micellar and vesicular PIC nanostructures are selectively formed depending on the ratio of the guanidine residues. Size-exclusion chromatography reveals that the encapsulation efficiency of CMS is dependent on the guanidinium modification ratio. The antimicrobial activity of the PIC nanostructures is also confirmed, indicating that the complexation of CMS in the PICs and further release from the PICs successfully occurs.
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U2 - 10.1002/marc.202200316
DO - 10.1002/marc.202200316
M3 - Article
C2 - 35661316
AN - SCOPUS:85131962433
SN - 1022-1336
VL - 43
JO - Macromolecular rapid communications
JF - Macromolecular rapid communications
IS - 19
M1 - 2200316
ER -