Mutants for rice storage proteins. III. Genetic analysis of mutants for storage proteins of protein bodies in the starchy endosperm

Two mutants for rice storage protein in the starchy endosperm, 13b-l. and 57-H, were crossed with their original variety, Kinmaze. From the results obtained in the F₁ and F₂ seeds, it was concluded that 13b-l. and 57-H are controlled by single recessive genes, which were designated as esp-1 and esp-2, respectively. The SDS-PAGE indicates that both mutant genes are regulatory genes for the protein bodies. The segregation ratio in F₂ of the cross between esp-1 and trisomic type F and the cross between esp-2 and trisomic type G fitted that of a trisomic segregation. These results suggest that esp-1 and esp-2 are located on chromosomes 10 and 9, respectively.